Fig. 1: Overexpression of Wt1–KTS led to the transformation of Leydig to granulosa-like cells. | Cell Discovery

Fig. 1: Overexpression of Wt1–KTS led to the transformation of Leydig to granulosa-like cells.

From: The −KTS isoform of Wt1 induces the transformation of Leydig cells into granulosa-like cells

Fig. 1: Overexpression of Wt1–KTS led to the transformation of Leydig to granulosa-like cells.The alternative text for this image may have been generated using AI.

a Histological analysis of gonads from control males, Wt1+/Ctg;Sf1-Cre males, and control females at 2 months of age. b Immunostaining of WT1, AMH, FOXL2, and CYP17A1 on control males, Wt1+/Ctg;Sf1-Cre males, and control females at E12.5. c–e The mRNA levels of Sertoli cell-specific genes (c), granulosa cell-specific genes (d), and Leydig cell-specific genes (e) in control testes, Wt1+/Ctg;Sf1-Cre testes and control ovaries at E12.5 (n = 6). f The expression of WT1, SOX9, CYP17A1, α-SMA, and FOXL2 in gonads of control males, Wt1+/Ctg;Sf1-Cre males, and control females at E16.5 was examined by immunofluorescence. g Schematic diagram illustrating the proposed role of Wt1 in regulating gonadal somatic cell differentiation. Data are presented as mean ± SEM. Two-tailed Student’s t-test. *P < 0.05; **P < 0.01.

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