Fig. 1: A spatiotemporal transcriptomic atlas of mouse placentation.

a Schematic illustration of the workflow for this study. Sections of mouse uterine segments were collected at time points ranging from E7.5 to E14.5 for Stereo-seq. b UMAP plot showing bin 50 of the labyrinth, JZ, and decidua from eight placental sections at six stages. Bins are colored according to placental structure or subregion annotations that were established by unsupervised Louvain clustering and manual annotation steps. c E7.5 S1, E8.5 S1, E9.5 S1, E10.5 S1, E12.5 S1, and E14.5 S1 uterine sections are shown with 24 annotated subregions together with the myometrium. Arrows with one head, arrows with two heads and arrows with three heads indicate the inner EPC, the early interface (EPC inva.) and the early interface (P-TGC inva.), respectively. Bin colors indicating subregion annotations are the same as in b. LaTP labyrinth trophoblast progenitor, SynT syncytiotrophoblasts, CP chorionic plate, EPC ectoplacental cone, JZ junctional zone, GlyT glycogen trophoblast, SpT spongiotrophoblast, SPA-TGC spiral artery-associated TGC, C-TGC canal TGC, P-TGC parietal TGC, MD mesometrial decidua, VSZ vascular sinuses zone, AMD anti-mesometrial decidua. d Multicolor display showing the E10.5 S1 section; seven well-known markers and a ssDNA layer are indicated. e Quantification of the proportion of each subregion in the labyrinth and JZ captured at each developmental stage. All 13 uterine sections were used in this analysis. Colormaps are shown as in c. f Spatial visualization of the expression of the indicated genes in the placenta from E7.5 to E14.5.