Fig. 6: Effect of high-fat diet consumption on regulation at the maternal–fetal interface.

a Schematic diagram showing the HFD treatment schedule and time points for sampling. b UMAP representation of 20 factors in the CD and HFD groups at E14.5. c Spatial distributions of factors 1, 8, 17, and 18 are shown, and colors from light blue to red indicate low to high coefficients for each bin50. d Proportions of immune cell types at the isolated maternal–fetal interface; E10.5 S1, E10.5 S2, E10.5 S3, H10.5 S1, E14.5 S1, E14.5 S2, and H14.5 S1 sections were used for this analysis. e Functional enrichment based on the top 50 weighted genes associated with factors 1, 8, 17, and 18. f Top ten genes defining the indicated NNMFs (factors). g RT-qPCR analysis of the expression of genes associated with factor 1 (Scgb1a1 and S100a8), factor 8 (Jam2 and Thbs1), factor 18 (Isg15 and Ifit3) and factor 17 (Pgf and Cd109). h Genes that were differentially expressed in the decidual and JZ of the maternal–fetal interface are shown. i Gene expression level and regulon activity of Irf7 in the decidua of the interface region. j Top 15 target genes of Irf7 in the CD and HFD groups at E14.5 are shown, and genes that were associated with factor 18 are labeled in red.