Fig. 5: SC mice with maternal transmission of H19-DMR deletion grow normally to adult.
From: Generation of live mice from haploid ESCs with germline-DMR deletions or switch
a Schematic diagram showing generation of SC embryos (H19-IG embryos) carrying maternally-derived H19-DMR deletion. H19-DMR was deleted in oocytes, which derived from H19-KO mice. IG-DMR was deleted in RFP AG-haESCs (RFP-IG-KO). Reconstructed embryos were obtained by intracytoplasmic AG-haESCs injection (ICAHCI). b A comparison of the development rates of E19.5 WT-IG, WT-DKO and H19-IG embryos. Viable status is judged by the breath. Data were generated from Table 2. c Fetal and placental weights of E19.5 WT-DKO (n = 12) and H19-IG (n = 8) embryos. Data are presented as mean ± SD, analyzed by Student’s t-test, ns, not significant. d, e Expression analysis of H19 (d) and Igf2 (e) in placenta, liver and brain of P1 WT-DKO and H19-IG pups (n = 3 for each group). Data are presented as mean ± SD, analyzed by Student’s t-test, *P < 0.05, ns, not significant. f–i Igf2 5’ UTR DNA sequences of PCR products amplified from DNA of RFP-DKO and RFP-IG-KO AG-haESCs (f), tail DNA of WT and H19-KO mouse (g), tail DNA of WT-DKO and H19-IG mouse (h), liver cDNA of WT-DKO and H19-IG mouse (i). j Relative allelic expression of the Igf2, as measured by Igf2 5’ UTR SNP analysis from placenta and liver cDNA of WT-DKO and H19-IG pups. k Growth curve of WT-DKO (n = 5) and H19-IG (n = 5) mice. l Survival curve of WT-DKO (n = 14) and H19-IG (n = 23) mice.
