Table 1 Summary of reconstructed embryos obtained from Ha-Ha-fusion-derived ESCs.
From: Generation of live mice from haploid ESCs with germline-DMR deletions or switch
2N fused ESC line* | No. of Transferred Embryos (TE) | No. of Observed Embryos (% TE) | No. of Alive Embryos (% TE) | No. of Transferred Embryos (TE) | No. of Observed Embryos (% TE) | No. of Alive Embryos (% TE) | No. of Transferred Embryos (TE) | No. of Observed Embryos (% TE) | No. of Alive Embryos (% TE) |
|---|---|---|---|---|---|---|---|---|---|
Embryo stage | E9.5 | E10.5 | E11.5 | ||||||
WT-DKO P30 | 72 | 29 (40.3) | 13 (18.1) | 60 | 11 (18.3) | 0 | NDa | ND | ND |
Xist-DKO | 33 | 21 (63.6) | 8 (24.2) | 66 | 31 (47.0) | 4 (6.1) | ND | ND | ND |
XI-DKO | ND | ND | ND | 34 | 13 (38.2) | 10 (29.4) | 34 | 8 (23.5) | 6 (17.6) |
Embryo stage | E13.5 | E14.5 | E15.5 | ||||||
XI-DKO | 32 | 4 (12.5) | 0 | ND | ND | ND | ND | ND | ND |
XIK-DKO | 168 | 15 (8.9) | 6 (3.6) | 76 | 2 (2.6) | 0 | ND | ND | ND |
XIKNP-DKO | 87 | 7 (8.0) | 3 (3.4) | 120 | 19 (15.8) | 3 (2.5) | 52 | 7 (13.5) | 0 |
XIKGPN-DKO | 35 | 3 (8.6) | 2 (5.7) | 110 | 2 (1.8) | 2 (1.8) | 96 | 4 (4.2) | 1 (1.0) |
Embryo stage | E17.5 | Full-Term | Â | ||||||
XIKGPN -DKO | 90 | 8 (8.9) | 0 | ND | ND | ND | |||
7KO-DKO | 16 | 2 (12.5) | 2 (12.5) | 136 | 10 (7.4) | 7 (5.1) | |||
8KO-DKO | ND | ND | ND | 322 | 37 (11.5) | 24 (7.5)b | |||
WT-DKO P8 | 34 | 7 (20.6) | 7 (20.6) | 51 | 8 (15.7) | 8 (15.7)c | |||
