Fig. 5: RelB suppresses type I IFN signaling and pro-inflammatory genes via its DNA binding function.

a Heatmap of z-scored CPM of all RelB^−/− hyper-expressed genes in WT, RelB^−/−, RelB^DB/DB, IFNAR^−/−, and IFNAR^−/−RelB^−/−, IFNAR^−/−RelB^DB/DB BMDCs. Genes selected for CpG (0.1 µM) induced (log₂FC > 1) in WT or RelB BMDCs at any time point and hyper-expressed (FC > 1.5) in RelB^−/− BMDCs relative to WT BMDCs at any time point (271 genes). Each row represents individual genes, and each column is from an individual time point upon CpG stimulation. b Top result of known motif analysis for gene clusters from a. Motif analysis considered –1 kb to +1 kb with respect to the TSS, GO results for gene clusters from a. c Line graphs of gene expression (CPM) for IFN-β and ISGs upon CpG stimulation (0 h, 1 h, 3 h, and 8 h). A dark blue line (circle) represents WT BMDCs, a dark red line (square) represents RelB^−/− BMDCs, dark green line (triangle) represents RelB^DB/DB BMDCs, light blue line (inverted triangle) represents IFNAR^−/− BMDCs, light red line (diamond) represents IFNAR^−/−RelB^−/− BMDCs, and light green line (circle) represents IFNAR^−/−RelB^DB/DB BMDCs. d Line graphs of gene expression (CPM) for IFN-independent hyper-expressed genes during CpG stimulation (0 h, 1 h, 3 h, and 8 h). A dark blue line (circle) represents WT BMDCs, a dark red line (square) represents RelB^−/− BMDCs, a dark green line (triangle) represents RelB^DB/DB BMDCs, the light blue line (inverted triangle) represents IFNAR^−/− BMDCs, light red line (diamond) represents IFNAR^−/−RelB^−/− BMDCs, and light green line (circle) represents IFNAR^−/−RelB^DB/DB BMDCs.