Table 1 Key materials and software.
Reagent or resource | Source | Identifier |
|---|---|---|
Antibodies | ||
Primary antibody against PPARα | Abcam Co. Ltd., CA, USA | ab126285 |
Primary antibody against ACADM | Proteintech Group, Inc., Wuhan, China | 55210-1-AP |
Primary antibody against ACADL | Proteintech Group, Inc., Wuhan, China | 17526-1-AP |
Primary antibody against CYP4A14 | Abcam Co. Ltd., CA, USA | ab140635 |
Primary antibody against CYP7A1 | Santa Cruz Biotechnology Inc., CA, USA | sc-518007 |
Primary antibody against CYP8B1 | Abcam Co. Ltd., CA, USA | ab236607 |
Primary antibody against CYP27A1 | Proteintech Group, Inc., Wuhan, China | 14739-1-AP |
Primary antibody against ACSL4 | Proteintech Group, Inc., Wuhan, China | 22401-1-AP |
Primary antibody against ACSL5 | Proteintech Group, Inc., Wuhan, China | 15708-1-AP |
Primary antibody against SCP2 | Abcam Co. Ltd., CA, USA | ab140126 |
Primary antibody against EHHADH | Proteintech Group, Inc., Wuhan, China | 26570-1-AP |
Primary antibody against β-actin | Proteintech Group, Inc., Wuhan, China | 81115-1-RR |
Goat secondary antibody against rabbit | Proteintech Group, Inc., Wuhan, China | RGAR001 |
Goat secondary antibody against mouse | Proteintech Group, Inc., Wuhan, China | RGAM001 |
Primary antibody against GLUT2 | Abcam, Ltd., Shanghai, China | ab152680 |
Primary antibody against GLUT4 | Abcam, Ltd., Shanghai, China | ab33780 |
Primary antibody against p-IR-β | Cell Signaling Technology, Inc., Shanghai, China | #2381 |
Primary antibody against p-IRS | Cell Signaling Technology, Inc., Shanghai, China | #3026 |
Primary antibody against IR-β | Cell Signaling Technology, Inc., Shanghai, China | #3025 |
Primary antibody against IRS | Cell Signaling Technology, Inc., Shanghai, China | #2382 |
Recombinant protein and chip | ||
Human PPARα Protein | Sino Biological Inc., Beijing, China | 12080-H07E |
Human PPARβ/δ Protein | Cloud-clone corp., Wuhan, China | RPC041Hu01 |
Human PPARγ Protein | Sino Biological Inc., Beijing, China | 12019-H20B |
Insulin | Beijing Solarbio Science & Technology Co., Ltd., Beijing, China | I8830 |
CM5 sensor chip | GE Healthcare, Chicago, IL, USA | BR100012 |
Experimental models: cell lines | ||
HepG2 (human hepatocellular carcinoma) cells | Procell Life Science&Technology Co., Ltd., Wuhan, China | CL-0103 |
C57BL/6J mouse | Charles River Biotechnology Co., Ltd, China | 213 |
Wistar rat | Charles River Biotechnology Co., Ltd, China | 102 |
GF C57BL/6J mouse | Gempharmatech Co., Ltd | 750 |
Critical commercial assays | ||
Dual Luciferase Reporter Gene Assay kits | Hanbio Biotechnology Co. Ltd., Shanghai, China | 20231017GX-DLR01 |
PPARα pcDNA3.1 plasmid | Hanbio Biotechnology Co. Ltd., Shanghai, China | 20231017GX-PC04 |
PPRE pGL4.26 plasmid | Hanbio Biotechnology Co. Ltd., Shanghai, China | 20231017GX-PC03 |
pRL-TK plasmid | Hanbio Biotechnology Co. Ltd., Shanghai, China | 20231017GX-PC06 |
Rat rxra pcDNA3.1-3xFlag-T2A-EGFP | Hanbio Biotechnology Co. Ltd., Shanghai, China | 20231017GX-PC02 |
Sodium palmitate/sodium oleate kit | Kunchuang Biotechnology, Xian, China | 2 |
PPARα siRNA | Hanbio Biotechnology Co. Ltd., Shanghai, China | 20230809GX-SI02 |
Control siRNA | Hanbio Biotechnology Co. Ltd., Shanghai, China | 20230809GX-SI02 |
Lipo 8000 transfection reagent | Beyotime Biotechnology, Shanghai, China | C0533 |
Insulin ELISA Kit for | Cloud-clone corp., Wuhan, China | CEA448Mu |
Opti-MEM | Invitrogen Co., Carlsbad, CA, USA | 31985070 |
Oil Red O Stain Kit | Solarbio Co. Ltd., Beijing, China | G1262 |
Mouse GPT/ALT kit | Grace Biotechnology Co. Ltd., Suzhou, China | G0423W |
Mouse GOT/AST kit | Grace Biotechnology Co. Ltd., Suzhou, China | G0424W |
Mouse TG Content Assay Kit | Grace Biotechnology Co. Ltd., Suzhou, China | G0910W |
Mouse TC Content Assay Kit | Grace Biotechnology Co. Ltd., Suzhou, China | G0909W |
Taq Pro Universal SYBR qPCR master mix | Vazyme Biotechnology Co. Ltd., Nanjing, China | Q712-02 |
E.Z.N.A.â Soil DNA kit | Omega Bio-Tek Co. Ltd., Norcross, GA, USA | D5625-02 |
HE Staining Kit | Solarbio Co. Ltd., Beijing, China | G1120 |
HiScript® III All-in-one RT SuperMix Perfect for qPCR | Vazyme Biotechnology Co. Ltd., Nanjing, China | R333 |
Software and algorithms | ||
BiacoreTM Insight Software | Cytiva | |
ImageJ | National Institutes of Health (NIH) | |
Adobe Illustrator | Adobe | |
SPSS 16.0 | IBM SPSS | |
R studio | RStudio | |
Graphpad Prism 7 | GraphPad | |
Bacterial and Chemicals | ||
Flavonifractor plautii | Guangdong Microbial Culture Collection Center (GDMCC) | GDMCC NO.1.1268 |
Phytosphingosine (≥ 98%) | Aladdin | D136389 CAS: 554-62-1 |
neomycin sulfate (600 μg/mg) | Macklin | N814740 CAS: 1405-10-3 |
metronidazole (≥ 98%) | Macklin | M813526 CAS: 443-48-1 |
Ampicillin (≥ 98%) | Macklin | A830931 CAS: 69-53-4 |
Vancomycin (≥ 950 μg/mg) | Macklin | V871983 CAS: 1404-90-6 |
Recombinant Human Insulin | Solarbio Co. Ltd., Beijing, China | I8830 |
TRIzol | Vazyme Biotechnology Co. Ltd., Nanjing, China | #R411 |
Streptozotocin | Sigma‒Aldrich, Inc., Shanghai, China | S0130 |
Other | ||
Automatic Biochemistry Analyzer | Beckman Coulter, Inc. CA, USA | AU480 |
Glucometer | Bayer, Leverkusen, Germany | D2ASCCONKIT |
Normal chow diet | SiPeiFu (Beijing) Biotechnology Co., Ltd, China | SPF-F02-003 |
High fat Diet | SiPeiFu (Beijing) Biotechnology Co., Ltd, China | 010139 |
High fat Diet With 60 kcal% diet | SYSE BIO Co., Ltd, Changzhou, China | PD6001 |
