Fig. 2: Identification and characterization of spermatogenic cell subpopulations.
From: Spatiotemporal single-cell architecture of gene expression in the Caenorhabditis elegans germ cells
a Re-clustering of the spermatogenic cell, shown in t-SNE. The fraction of the total cells in each subset is as follows: SP1 (1210), SP2 (2231), SP3 (1831), SP4 (1992) and SP5 (1621). b Dot plot showing the relative expression of marker genes in each inferred cell type. The size of each dot refers to the proportion of cells expressing a gene, and the color of each dot represents the calculated scaled expression value; gray is the lowest, and red is the highest. Red indicates known markers. Black indicates identified DEGs. c Heatmap of differential gene expression in the five subsets. Shown is each gene’s mean expression in each cell type, standardized over the five centroids. Shown on the right are representative enrichments of biological processes. d Examples of new marker genes. Scale bar, 20 μm.
