Fig. 2: The structures of HBsAg_{Type A} and HBsAg_{Type B}.

a Side view of the cryo-EM map of the HBsAg–Fab_HBC complex. HBsAg-A, HBsAg-B, Fab_HBC-heavy chain variable region (VH), and Fab_HBC-light chain variable region (VL) are colored in green, purpleblue, pink, and orange, respectively. The sizes of the HBsAg and AGL are labeled. The detergent micelle and unresolved flexible region are shown in semi-transparent gray. The approximate boundaries of the phospholipid bilayer are indicated as thick gray lines. b A 90° rotated top view of a. c Side view of the cryo-EM map of the HBsAg–Fab_H006 complex. HBsAg-A subunit and HBsAg-B subunits are colored the same as in (a), while Fab_H006-VH and Fab_H006-VL are colored in steel blue and light blue, respectively. The approximate boundaries of the outer leaflet of the viral envelope are indicated as thick gray lines. d A 90°-rotated top view of c. e Top view of the AGL_{Type A} in cartoon representation. AGL-A and AGL-B are in the same colors as in (a). β-strands and loops are indicated. f 2D topology diagram of AGL_{Type A}. β-strands and loops are indicated. Flexible regions that are not resolved are denoted with dashed lines. g Top view of the AGL_{Type B} in cartoon representation. AGL-A and AGL-B are in the same colors as in (c). h 2D topology diagram of AGL_{Type B}. i Side view of the AGL_{Type A} in cartoon representation. Cysteine pairs forming disulfide bonds are indicated, and the disulfide bonds are highlighted in yellow. j Side view of the AGL_{Type B} in cartoon representation. k Schematic diagram of AGL_{Type A}. Cysteines are indicated by yellow circles with their residue numbers. The intramolecular disulfide bonds are labeled in blue, and the intermolecular disulfide bonds in orange. Flexible regions that are not resolved are denoted with dashed lines. l Schematic diagram of AGL_{Type B}.