Fig. 6: Novel lipid regulation of GPR4.
From: Molecular mechanism of pH sensing and activation in GPR4 reveals proton-mediated GPCR signaling
a–c The overall view of LPC-binding sites around GPR4–Gs at pH 6.5 (a) and pH 7.4 (b), and those around GPR4–Gq at pH 7.4 (c), respectively. LPCs and water molecules are shown as sticks and red balls, respectively. d The engaged residues between LPCs and TM3/5 of GPR4. The polar interactions are displayed by orange dashed lines. e The effect of S200A mutation on LPC positive allosteric activity measured by pH-induced cAMP accumulation in GPR4 WT or mutants with or without 100 μM LPC. Values are represented as means ± SEM of three independent experiments (n = 3). f, g Structural superposition of GPR4 and ADHRF1 (PDB: 7WU3).
