Fig. 1: Age-related neuroinflammation drives MT1-MMP activation in the hippocampus.
From: MT1-MMP inhibition rejuvenates ageing brain and rescues cognitive deficits in obesity
a Immunoblotting of MT1-MMP from hippocampal lysates of 2-month-old (young) and 18-month-old (aged) mice (n = 2 mice per group). b Quantification of MT1-MMP expression relative to β-actin loading control (n = 5 mice per group). Two-tailed unpaired Student’s t-test. c MMP14 activity was measured as relative fluorescence units (RFU) in the hippocampus sample of young and aged mice. Two-tailed unpaired Student’s t-test (n = 5 mice per group). d MMP14 normalized transcript per million (nTPM) data from adult genotype tissue expression (GTEx) portal and comparative analysis between young and aged human hippocampus tissue. Two-tailed unpaired Student’s t-test. e Hippocampal Mmp14 mRNA levels by qPCR in young, aged, and C52-treated aged mice. One-way ANOVA followed by Tukey’s multiple comparisons test. (n = 5 young, n = 6 aged, n = 6 aged + C52 mice). f–m Correlation analysis between MMP14 and pro-inflammatory factors NF-κB (f), IL-1B (g), IL-6 (h), CCL3 (i), IL23A (j), CD11B (k), TNF (l) and C1QB (m) from the RNA sequencing data from human hippocampus tissues obtained through GTEx portal. Data are expressed as mean ± SEM, and each data point represents individual mouse/human.
