Fig. 5: Ectopic expression of MT1-MMP in the hippocampus induces cognitive deficit in 2-month-old young mice.

a Percentage preference for the displaced object in the object location task from mice receiving either AAV-control, AAV-WT MT1-MMP, or AAV-MT1 EA. One-way ANOVA followed by Tukey’s multiple comparisons test (n = 8 mice per group). b, c Percentage of total time spent in the target quadrant (b) and distance traveled to the target hole (c) in the Barnes maze test. One-way ANOVA followed by Tukey’s multiple comparisons test (n = 5 mice per group). d Percentage of freezing time during a contextual fear conditioning test. One-way ANOVA followed by Tukey’s multiple comparisons test (n = 6 mice per group). e, f Changes in fEPSP in the hippocampal CA1 for LTP analysis (e) and the average of fEPSP (f). One-way ANOVA followed by Tukey’s multiple comparisons test (n = 6 mice per group). g–i Young and aged mice treated with C52 (10 mg/kg/day) for two weeks after AAV injection and tested for spatial memory using object location task (g) and Barnes maze test (h, i). One-way ANOVA followed by Tukey’s multiple comparisons test (n = 6 mice per group). Data are expressed as mean ± SEM, and each data point represents individual mouse.