Fig. 2: OsVIRK1 co-localizes with the TGN/EE marker SCAMP1. | Cell Discovery

Fig. 2: OsVIRK1 co-localizes with the TGN/EE marker SCAMP1.

From: A receptor-like kinase recognizes viral proteins at the trans-Golgi network/early endosome and inhibits infection in rice

Fig. 2

a, c 35S promoter-driven OsVIRK1-GFP was transiently co-expressed with SCAMP1-RFP in rice protoplasts (a) and N. benthamiana leaves (c). b, d OsVIRK1 native promoter-driven OsVIRK1-GFP was transiently co-expressed with SCAMP1-RFP in rice protoplasts (b) and N. benthamiana leaves (d). The GFP and RFP fluorescence signals were observed by confocal laser microscopy. Arrows indicate representative fluorescence signals of puncta. Scale bars, 10 μm. e Membrane fractionation assay showing the colocalization of OsVIRK1-FLAG-HA and SCAMP1-RFP in the plasma membrane and vesicle fractions. These fractions were prepared from protoplasts of OsVIRK1-FLAG-HA-overexpressing rice seedlings transiently expressing SCAMP1-RFP. Western blot was performed with anti-HA, anti-RFP, or anti-H+-ATPase antibody. All experiments were repeated two or three times with similar results.

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