Fig. 3: The helicase subunit UL5 adopts a fully open conformation.
From: Structural and mechanistic insights into the herpes simplex virus type 1 helicase-primase primosome
a Cartoon representation of UL5 colored by domain (schematic). Domains 1A, 2A, and 2B are explicitly highlighted. b Cartoon representation of Thermus oshimai Pif1 (ssDNA/ADP-bound; PDB: 6S3O). Domains 1A, 2A, and 2B are explicitly highlighted. c Superposition of Pif1 onto UL5 highlights divergent conformations in the 2A and 2B domains. d Close structural alignment of the 1A domains and ADP-binding sites between UL5 and Pif1. e Conformation of the Pif1 2A–2B domains. f Conformation of the UL5 2A–2B domains. g Domain 2A in UL5 exhibits an ~20° rotation relative to Pif1. UL5 domain 2B lacks the 1A-proximal loop observed in Pif1.
