Fig. 3: Effects of epigenetic features and chromatin accessibility on the off-target activity of Cas9 variants.
From: Decoding the role of chromatin context in the off-target effects of CRISPR gene editing with EGOLD
a Percentage of off-target types with significant and non-significant associations. Off-target types were considered “influenced” by chromatin context if they were significantly associated with any specific epigenetic feature or chromatin accessibility (P < 0.05, Wilcoxon test); the minimum off-target number for each type was ≥ 10. b Heatmap of P values for different epigenetic or chromatin accessibility features in correlation analyses with edited and unedited off-target sites for each gene editor (P < 0.05, Wilcoxon test). Red reflects positive correlations; blue reflects negative associations. c Relationships between off-target effects and different epigenetic features or degrees of chromatin accessibility for each Cas9-variant gene editor (P < 0.05, Wilcoxon test). Red reflects positive correlations; blue reflects negative associations.
