Fig. 1
From: Structure of the receptor-activated human TRPC6 and TRPC3 ion channels
Characterization of the high-affinity inhibitor BTDM. a Chemical structure of the BTDM used in this study. b Whole-cell currents of hTRPC6 evoked by OAG were inhibited by BTDM in sub-IC50 concentration. Voltage ramps were applied every 10 s, and the outward current at +100 mV and inward current at −100 mV during each ramp are plotted as a function of time after rupture of the patch for whole-cell recording. Solid and hollow circles represent currents at −100 mV and +100 mV, respectively. Solution exchanges were indicated by horizontal bars. I–V curves were obtained during voltage ramps at the time points indicated. The inhibitor could inhibit hTRPC6 currents with the inhibition rate of 45.54% ± 2.22% at 2 nM (n = 3). c Activation effect of OAG on hTRPC6 constructs, measured by FLIPR membrane potential assay (n = 3). d Inhibition effect of the BTDM on wild-type hTRPC6 (n = 3)
