Fig. 5

Prrc2a modulates oligodendroglia fate determination, proliferation, and differentiation in vitro. a Immunostainings of Pdgfrα/Olig2 or GFAP in oligosphere-derived cells 14 days postBl04-CM treatment from mice with indicated genotypes. b Quantification of the percentage of Pdgfrα⁺Olig2⁺ and GFAP⁺ cells from mice with indicated genotypes (one-way ANOVA followed Tukey test, **P < 0.01, n = 4 each group). c Gene expressions during neurosphere to oligosphere transformation in cells 14 days post B104-CM treatment. The gene expressions were normalized to those in wild-type neural stem cells (one-way ANOVA followed Tukey test, **P < 0.01, ***P < 0.001, n = 4 per group). d Immunostainings of Pdgfrα and Sox10 in spinal cord from mice with indicated genotypes at E12.5 and E14.5. e The quantification of the number of OPCs (Pdgfrα and Sox10 double positive) in spinal cord from mice with indicated genotypes at E12.5 and E14.5 (one-way ANOVA followed Tukey test, *P < 0.05, **P < 0.01, ***P < 0.001, n = 6 per group). f Immunostainings of Pdgfrα/Olig2/BrdU in the cultured OPCs after incubation with BrdU (50 μg/ml) for 2 h. The bar graph (g) depicts the quantification of the percentage of BrdU⁺ Pdgfrα⁺ Olig2⁺ cells (one-way ANOVA followed Tukey test, *P < 0.05, n = 4 per group). h BrdU (50 mg/kg) was intraperitoneally injected into P6 mice. 2 h later, the mice were sacrificed and the brain sections of corpus callosum were immunostained with anti-Pdgfrα and anti-Ki67 (upper panel) or anti-BrdU (bottom panel) antibodies. Arrowheads indicate the proliferating OPCs (Pdgfrα and Ki67/BrdU double-positive cells). iThe quantification of the number and percentage of proliferating OPCs from mice with indicated genotypes (one-way ANOVA followed Tukey test, *P < 0.05, ***P < 0.001, Pdgfrα⁺Ki67⁺: n = 6 each group; Pdgfrα⁺BrdU⁺: n = 7 each group; Proliferation OPCs Pdgfrα⁺BrdU⁺/Pdgfrα⁺: n = 7 each group). j Immunostainings of O4/Olig2 and Mbp/Olig2 in oligodendrocytes after 3 days of T3 treatment. The bar graph (k) depicts the quantification of the percentage of O4⁺ Olig2⁺ and Mbp⁺Olig2⁺ cells (one-way ANOVA followed Tukey test, **P < 0.01, ***P < 0.001, n = 4 per group). l Gene expressions of T3-induced differentiated oligodendrocytes from mice with indicated genotypes (one-way ANOVA followed Tukey test, ***P < 0.001, n = 4 per group)