Fig. 3
From: Structural insights into a high fidelity variant of SpCas9
Structural rearrangement of the REC lobe of xCas9 3.7. a Superimposition of the RNA/DNA heteroduplex-bound REC lobe of SpCas9 (PDB: 4UN3) and xCas9 3.7. Color codes are the same as those shown in Fig. 1a. The six residues in REC lobe substituted in xCas9 3.7 are highlighted in red. b A close-up view of the detailed interactions of REC domains of SpCas9 (shown in left) and xCas9 3.7 (shown in right). Black dashed lines represent hydrogen bonds. c A close-up view of the detailed interactions between REC3 and RNA/DNA heteroduplex of SpCas9 (shown in left) and xCas9 3.7 (shown in right). d Quantification of in vitro cleavage activity of xCas9 3.7 variants using substrates containing different PAMs. n = 3. Error bars represent S.D
