Fig. 4
From: Structural insights into a high fidelity variant of SpCas9
Structure-based design of high-fidelity SpCas9 variants. a In vitro cleavage assay showing the substrate specificity of SpCas9 variants. Activities of rationally designed SpCas9 variants using fully matched or doubly mismatched DNA substrates with 5′-TGG-3′ PAM. n = 3. Error bars represent S.D. b In vitro cleavage assay showing the substrate specificity of SpCas9 variants. Activities of rationally designed SpCas9 variants using fully matched or doubly mismatched DNA substrates with 5′-TGT-3′ PAM. n = 3. Error bars represent S.D. c Mutations at PAM recognition sites increase SpCas9 substrate specificity. Left: Zoom-in view of the 5′-TGG-3′ PAM DNA recognition by SpCas9. Right: Activities of structure-based mutagenesis of SpCas9 (see left) for fully matched or doubly mismatched DNA substrates containing 5′-TGG-3′ PAM. n = 3. Error bars represent S.D
