Abstract
The transient elevation of cytoplasmic calcium is essential for pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI). However, the calcium channels responsible for this process have remained unknown. Here, we show that rice CDS1 (CELL DEATH and SUSCEPTIBLE to BLAST 1) encoding OsCNGC9, a cyclic nucleotide-gated channel protein, positively regulates the resistance to rice blast disease. We show that OsCNGC9 mediates PAMP-induced Ca2+ influx and that this event is critical for PAMPs-triggered ROS burst and induction of PTI-related defense gene expression. We further show that a PTI-related receptor-like cytoplasmic kinase OsRLCK185 physically interacts with and phosphorylates OsCNGC9 to activate its channel activity. Our results suggest a signaling cascade linking pattern recognition to calcium channel activation, which is required for initiation of PTI and disease resistance in rice.
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Acknowledgements
We thank Drs. Guo-Liang Wang, Yuese Ning and Xuetao Shi for technical assistance in ROS detection; Drs. Xiaojuan Zhang, Weibing Wang and Jiafan Liu for help in phosphorylation assay. This work was supported by the National Key R&D Program of China (2017YFD0100404 and 2016YFD0100600) and National Natural Science Foundation of China (31471758).
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Jiachang W., X.L. and A.Z. designed and performed most of the experiments. Y.X., Fan W. and Yihua W. performed genetic analysis and mapping. Yan-Qiu T. and Yong-Fei W. performed the electrophysiological assay. C.L., J.L., S.W., L.M. and H.Z. performed blast inoculation and subcellular localization assay. T.P., Yongfei W. and C.Z. performed protein–protein interaction assay. Xin Z., X.G., L.J., Yupeng W., X.J., Sheng L. and Xiao Z. performed construction and genetic transformation. G. W., X.C. and E. W. constructed the Osrlck185/55 double mutant plants. S.Z., Y.R., Q.L., C.W., Z.C., Jiulin W., Yunlu T. and Shijia L. provided technical help with experiments. Jiachang W., Y.R. and Fuqing W. drafted the manuscript. Jianmin W., J.Z. and H.W. supervised the project and revised the manuscript.
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Wang, J., Liu, X., Zhang, A. et al. A cyclic nucleotide-gated channel mediates cytoplasmic calcium elevation and disease resistance in rice. Cell Res 29, 820–831 (2019). https://doi.org/10.1038/s41422-019-0219-7
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DOI: https://doi.org/10.1038/s41422-019-0219-7
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