Fig. 2: Molecular recognition of GLP-2 by GLP-2R.
From: A unique hormonal recognition feature of the human glucagon-like peptide-2 receptor
a Extensive contacts between GLP-2R (sticks with orange red carbons) and GLP-2 (sticks with forest green carbons), showing that the N-terminal residue His1P can interact with H2683.37b, V2713.40b and W3405.36b, together with other interactions between agonist and receptor (left). The interactions focus on the ECL1 of GLP-2R and GLP-2 (right). b, c Structural comparison of the TMD binding pockets among GLP-2–GLP-2R–Gs, GLP-1–GLP-1R–Gs,13 and glucagon–GCGR–Gs.19 GLP-2 in forest green, GLP-2R in orange red; GLP-1 in blue, GLP-1R in light blue; glucagon in magenta and GCGR in light magenta. Receptor ECD and G protein are omitted for clarity. d Effects of mutation in the peptide-binding pocket on cAMP accumulation. cAMP levels were measured in WT receptor and alanine-mutated TMs and ECLs. Data shown are means ± SEM of at least three independent experiments.
