Fig. 3: The properties of hotspot-associated H3K4me3 marks during meiotic recombination.

a Heatmaps of H3K4me3 normalized tag density on four types of hotspot-associated H3K4me3 peaks with distinct turnover times from leptotene to middle pachytene spermatocytes. Each row represents a DSB hotspot region of ±5 kb around the center. L leptotene, mZ mid-zygotene, lZ late-zygotene, e1P early 1-pachytene, mP mid-pachytene. b Boxplot showing the average width of the four subtypes of hotspot-associated H3K4me3 peaks in mid-zygotene. Definitions of the four subtypes of hotspot-associated H3K4me3 are detailed in Materials and Methods. c Profile of the averaged H3K4me3 tag density at mZ stage on the four subtypes of hotspot-associated H3K4me3 peaks. H3K4me3 tag density was calculated using H3K4me3 read coverage with 50-bp resolution. mZ mid-zygotene spermatocytes. d Identified PRDM9 motifs enriched on four subtypes of hotspot-associated H3K4me3 peaks.