Fig. 6: SSK1 improves physical function of aged mice.

a Quantification of maximal time in rotarod of old mice treated with vehicle (Veh) or SSK1 (0.5 mg/kg) compared with young (Y), middle-aged (M), old (O) mice control (n = 12, 13, 10, 9, 9 for each group, respectively). b Exhaustion distance in treadmill of old mice treated with vehicle or SSK1 compared with young, middle-aged, old mice control (n = 12, 13, 10, 10, 9 for each group, respectively). c Grip strength of four limbs of old mice treated with vehicle or SSK1 compared with young, middle-aged, old mice control (n = 12, 13, 9, 6, 7 for each group, respectively). d The number of rearing exploration times within 5 min of old mice treated with vehicle or SSK1 compared with young, middle-aged, old mice control (n = 12, 13, 10, 9, 7 for each group, respectively). e Time to cross balance beam for old mice treated with vehicle or SSK1 compared with young, middle-aged, old mice control (n = 12, 13, 10, 9, 9 for each group, respectively). f Serum biochemical test. The levels of Alanine transaminase (ALT), aspartate transaminase (AST), creatinine (CREA), uric acid (UA) and carbamide (UREA) in old mice after vehicle or SSK1 treatment for 8 weeks are shown (Vehicle-treated, n = 8; SSK1-treated, n = 8). g Routine analysis of blood. The numbers of granulocytes (GR), lymphocytes (LY), white blood cells (WBC), red blood cells (RBC), and platelets (PLT) of old mice after vehicle or SSK1 treatment for 8 weeks are shown (Vehicle-treated, n = 8; SSK1-treated, n = 8). Each data point represents an individual mouse. ‘n’ represents the number of mice. Data are presented as means ± SEM. Unpaired two-tailed t-test, *P < 0.05, **P < 0.01.