Fig. 6: Inhibition of Lysyl oxidase activity or Loxl2 reduces OS growth.

a Experimental procedure: 5-week-old H2-c-fosLTR mice were injected three times/week during 15 weeks with 250 mg/kg BAPN to block lysyl oxidase activity or with vehicle (PBS) and tumors were monitored longitudinally by Micro-CT. b Average tumor burden per mouse. c Volumetric follow-up over time for 20 and 18 tumors from PBS-treated and BAPN-treated mice, respectively at 5, 10, 15 and 20 weeks in PBS- or BAPN-treated mice. d, e Representative images of Picrosirius red/polarized light staining (d) and quantification of tumor collagen packing density (e) from tumor-bearing PBS- and BAPN-treated mice at 20 weeks. f–h Experimental procedure: 5-week old H2-c-fosLTR mice were injected two times/week during 15 weeks with anti-Loxl2 blocking antibody (30 mg/kg) or with IgG and tumors were monitored longitudinally by Micro-CT (f) for tumor number per mouse (g) and average tumor volume (h) at 5 and 20 weeks. Bar graphs and plots represent or include mean ± SEM, respectively. Black asterisks: *P < 0.05 and **P < 0.01 by two-way ANOVA with Bonferroni post hoc test. Red asterisks: *P < 0.05, **P < 0.01 and ***P < 0.001 by two-tailed t-test between PBS- and BAPN-treated at end point and all mice at start.