Fig. 6: MutLα interacts with DNA.
From: Mispair-bound human MutS–MutL complex triggers DNA incisions and activates mismatch repair
a–c Sucrose gradient centrifugation was performed to determine the molecular interactions between MutSα, MutLα, and heteroduplex DNA (a), MutSα and DNA (b), MutLα and DNA (c). Reaction mixtures were incubated on ice for 20 min, followed by centrifugation (16 h, 45,000 rpm, at 4 °C), fractionation (from the bottom to the top), electrophoresis, and western blotting.
