Fig. 1: MACOM directly binds RNA substrates and is essential for m6A writer activity. | Cell Research

Fig. 1: MACOM directly binds RNA substrates and is essential for m6A writer activity.

From: Cryo-EM structures of human m6A writer complexes

Fig. 1: MACOM directly binds RNA substrates and is essential for m6A writer activity.

a Model of RNA m6A methylation by MAC and MACOM. b Overlay view of size exclusion chromatography results of HWVZ, WVZ, HWV, and WV complexes. c SDS-PAGE of HWVZ, WVZ, HWV, and WV complexes, corresponding to b. d Binding affinities of the MAC, MAC + HWVZ, HWVZ, WVZ, HWV, and WV complexes with FAM-labeled ACTB-1 RNA from EMSA experiments. Binding curves were generated using the means of three independent experiments, with standard deviation (SD) values indicated by error bars (n = 3). e Overlay view of size exclusion chromatography results of MAC, HWVZ, and MAC-HWVZ complexes. f SDS-PAGE of MAC, HWVZ, and MAC-HWVZ complexes, corresponding to e. g ACTB-1 RNA N6-adenosine methylation activity of MAC with or without HWVZ, WVZ, HWV, and WV complex. Data are shown as the means ± SD (n = 2).

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