Fig. 2: Overview of human MACOM structure.
a Schematic illustration of the domain organization of proteins in the reconstituted human MACOM complex. The dash lines indicate regions that are invisible in the cryo-EM structure. b, c The 3.0-Å resolution cryo-EM map (left panel) and cartoon model (right panel) of HWVZ complex in two different views, with WTAP-a in orange, WTAP-b in yellow, ZC3H13 in orange-red, VIRMA Belly domain in dodger-blue, Chest domain in slate-blue, Back domain in cyan, Neck domain in cornflower-blue, Head domain in deep-sky-blue, and Halter domain in green. This color coding applies to the whole article. d Surface presentation of the HWVZ complex. The surface in the left panel is colored corresponding to a. The surface in the right panel is colored by electrostatic potential. e The 3.0-Å resolution cryo-EM map of HWV complex. f Overall structure of the HWV complex is shown in the same orientation as the cryo-EM map in e. g Surface presentation of the HWV complex. The left panel surface is colored corresponding to a. The right panel surface is colored by electrostatic potential. h Superposition of the HWVZ (colored) and HWV (dark gray) complexes, aligned by WTAP. i Conformational change of the HWV complex upon ZC3H13 binding, aligned by WTAP.
