Fig. 5: ZC3H13 interacts with VIRMA and induces conformational change of MACOM.

a Overview of the ZC3H13 Barding domain binding to the HWV complex. ZC3H13 is shown in cartoon mode. The WTAP homodimer and VIRMA are shown in translucent surface. b Details of the interface formed by Barding domain-α1, WTAP-a-H4, Chest and Belly domains of VIRMA, corresponding to blue box in a. c ARML3–4 in Belly domain of VIRMA and ARML-Z1-2 in Barding domain of ZC3H13. ARML repeats of the Belly domain are shown in different colors. ARML repeats Z1 (α2–α4) and Z2 (α5–α6) are colored in orange-red. d Inter-repeat interactions of ARML-Z1 in the ZC3H13 Barding domain and ARML-4 in the Belly domain. e, f Density map of α7 in the Barding domain and Halter domain interface with the corresponding atomic model from the HWVZ and HWV complexes, respectively. g Interactions between α8 in the Barding domain and Chest domain of VIRMA, corresponding to the light-green box in a. h Interactions between α6, α7 of the ZC3H13 Barding domain and Neck domain of VIRMA, corresponding to the forest-green box in a. i Conformational changes of the HWV complex upon ZC3H13 binding, aligned by the Chest domain of VIRMA. j Model of ZC3H13 α6 and α7 binding-induced conformational changes of the HWV complex. k Interactions between α3, α6 of the ZC3H13 Barding domain and Chest domain of VIRMA, corresponding to the cyan box in a.