Fig. 1: RASON is overexpressed in human pancreatic cancer.
From: A novel protein RASON encoded by a lncRNA controls oncogenic RAS signaling in KRAS mutant cancers
a Scatter plot showing the ribosome release score (RSS) and the ORF score from main ORF (mORF, blue) and lincORF (purple) (x-axis, Log10 RSS; y-axis, ORF score). The dashed lines represent the 95% percentile of RSS and ORF scores from mORF, which are set as the threshold to identify actively translated lincORF. Along each axis, all points are summarized using an overlaid density plot. b Venn diagram of differentially translated lincRNAs in three PDAC cell lines BxPC-3, PANC-1, and AsPC-1 compared to HPNE normal cells by Ribo-seq. c Upper, differential RPFs of RASON sORF between HPNE and PDAC cells; lower, diagram of the sORF encoding RASON. d Scheme of CRISPR/Cas9-induced FLAG-tag insertion after RASON sORF inside LINC00673 genomic DNA in 293T cells. e IB showing successful expression of RASON fused with the FLAG tag in d. f RASON protein expression in 293T cells transfected with LINC00673, RASON-ORF, RASON-ORF with ATG mutation, or sh-LINC00673. g, h Correlation analysis of RASON expression with tumor grade in 152 PDAC patients. Data are means ± SD. i Kaplan-Meier curve showing overall survival of patients stratified by RASON expression in the 152 PDAC patient cohort. j Representative IHC images of RASON expression in tumors from 105 PDAC patients, with blocking peptide showing the specificity of anti-RASON antibody (bars, 250 µm or 50 µm). k IB of RASON expression in a panel of PDAC cell lines. l RASON protein expression levels in paired cancerous and normal tissues (>2 cm away) from 14 randomly selected PDAC patients. Data in bar graphs are shown as means ± SD. P values were calculated by Wilcoxon test (g, h) and log-rank analysis (i). ***P < 0.001.
