Fig. 1: Schematic representation of RNA-guided and protease-based immunity by Craspase.
From: A type III-E CRISPR Craspase exhibiting RNase and protease activities
a Model of type III-A/B Csm/Cmr complex relies on target ssRNA to regulate ssRNA/ssDNA cleavage and cAn synthesis. b Model for gRAMP RNase activation upon target RNA loading. c Model for Craspase-mediated immunity. Invading RNA induces conformational changes of TPR-CHAT and thereby allosterically triggers peptidase activity on Csx30, resulting in cell death. For a–c, pairings with self and non-self RNAs are shown in left and right panels, respectively, while red wedges represent cleavage sites.
