Fig. 1: CAR-T exhaustion induced by high tonic signaling of many commonly used CARs.
From: Tuning charge density of chimeric antigen receptor optimizes tonic signaling and CAR-T cell fitness
a FACS analysis of CD69 expression level of Jurkat T cells expressing wild type and ITAM mutant CD19, CSPG4, and GD2.CARs. b CD69/GFP, defined as the tonic signaling index, of the three CARs shown in a. c The tonic signaling index of ten commonly used CARs. d The average expression of LAG3, TIM3 and PD-1 on CAR-T cells used to define the exhaustion score. e The exhaustion score of the CAR shown in c. f Correlation between the exhaustion score and the tonic signaling index in these CAR-T cells assessed by the Pearson method. Data are presented as means ± SEM. All comparisons were determined using Student’s t-tests; **P < 0.01; ****P < 0.0001.
