Fig. 7: Schematic diagram illustrating the NADase activation mechanisms of the TIR-APAZ/Ago and SIR2-APAZ/Ago systems. | Cell Research

Fig. 7: Schematic diagram illustrating the NADase activation mechanisms of the TIR-APAZ/Ago and SIR2-APAZ/Ago systems.

From: Structural insights into mechanisms of Argonaute protein-associated NADase activation in bacterial immunity

Fig. 7: Schematic diagram illustrating the NADase activation mechanisms of the TIR-APAZ/Ago and SIR2-APAZ/Ago systems.

Both systems utilize a conserved sensor loop in the PIWI domain of Ago protein to detect the formation of the gRNA–target ssDNA duplex, thereby initiating the conformational changes required for the activation of the NADase activity in TIR domain or SIR2 domain. While TIR domain oligomerization is needed to form the active pocket in TIR-APAZ/Ago system, the target ssDNA binding activates SIR2 domain by increasing flexibility.

Back to article page