Fig. 1: Technical pipeline of the TESOS method. | Cell Research

Fig. 1: Technical pipeline of the TESOS method.

From: Mapping of individual sensory nerve axons from digits to spinal cord with the transparent embedding solvent system

Fig. 1: Technical pipeline of the TESOS method.The alternative text for this image may have been generated using AI.

a Sample processing steps of the TESOS method. b Formation of the organogel is the conceptual basis for transparent embedding. c Relative mechanical strength of various samples displayed based on log10 relative Young’s modulus values (n = 3). Young’s modulus value of a freshly fixed brain was normalized as 1. d Representative images of 6-week-old Thy1-YFP-16 mice before clearing, after clearing and after polymerization. e Small samples were glued on a MagMount device and a rotary microtome was used for sectioning the surface. Large whole-body samples were directly glued within a chamber under a light sheet microscope for imaging in combination with a milling platform. f Data processing pipeline.

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