Fig. 3: Whole-body imaging of a mouse pup at micrometer resolution with a confocal microscope. | Cell Research

Fig. 3: Whole-body imaging of a mouse pup at micrometer resolution with a confocal microscope.

From: Mapping of individual sensory nerve axons from digits to spinal cord with the transparent embedding solvent system

Fig. 3

The body trunk of a Thy1-YFP-16 mouse pup at P5 was processed following the TESOS protocol and imaged with a 20×/0.95 NA immersion objective (gold, YFP signal; blue, autofluorescence) (voxel size, 0.9 × 0.9 × 3.5 µm3). a The final image stack of 3.5 cm (x) × 1.0 cm (y) × 1.8 cm (z) was stitched from 40 slabs with a thickness of ~500 μm each. b, c Image in a was re-sliced at a z-depth of 1 mm (b) or 6 mm (c). Boxed regions were enlarged to show details. b1 Spiral ganglion (spiral G.). Boxed region was enlarged to show axons innervating hair cells. b2 Optic nerve and retina. b3 Trigeminal ganglion (trigeminal G.). c1 DRGs at the level of the thoracic vertebrae. Boxed region was enlarged to show individual DRG (c2) and NMJs (c3). d–h Lateral view of the image stack (d). Boxed regions were re-sliced to show the thoracic DRGs (e), branchial plexus (f), T10 DRG (g) and the sciatic nerve (h).

Back to article page