Fig. 6: Sub-micron resolution imaging reveals complete projections of individual sensory neurons in the spinal cord. | Cell Research

Fig. 6: Sub-micron resolution imaging reveals complete projections of individual sensory neurons in the spinal cord.

From: Mapping of individual sensory nerve axons from digits to spinal cord with the transparent embedding solvent system

Fig. 6

DRG neurons were labeled by injecting Ai140 mouse pups (P6) with Synapsin-Cre AAV at the walking pad position. Cervical vertebrae were collected 2 months after injection and imaged with a 40×/1.3 NA objective (voxel size, 0.4 × 0.4 × 1.2 µm3; gold, GFP signal; blue, autofluorescence). a The final image stack of 2.5 × 3.8 × 6 mm3 dimension was constructed from 28 slabs with a thickness of 250–300 µm each. b Caudal view of the image stack. Boxed regions were enlarged and re-sliced to display details including DRG neurons (b1, b2). c Bifurcations of DRG neuron central branches. Boxed regions in c were enlarged to display an axonal arbor (c1) and boutons (c2). d A motor neuron that was randomly labeled. e Lateral view of the image stack. Boxed regions were enlarged to display DRG neurons (e1) and axons (e2). Dotted lines in e1 and e2 indicated boundaries between 2 stitched adjacent stacks in the z dimension. f Complete projection of 12 DRG neurons within the spinal cord.

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