Fig. 7: Mesoscale connectome mapping of individual sensory neurons from the digit hair follicles on the forepaw to the spinal cord. | Cell Research

Fig. 7: Mesoscale connectome mapping of individual sensory neurons from the digit hair follicles on the forepaw to the spinal cord.

From: Mapping of individual sensory nerve axons from digits to spinal cord with the transparent embedding solvent system

Fig. 7

An adult Thy1-EGFP-M mouse was processed following the TESOS method. The embedded sample was selectively imaged with a 40 × /1.3 NA objective on a confocal microscope (voxel size, 0.4 × 0.4 × 1.5 µm3). a The final image stack (25 × 18 × 20 mm3) was stitched from 98 slabs with a thickness of 250–350 µm each. The indicated regions are enlarged in the boxes. b Complete tracing of 5 sensory neurons from hair follicles to the spinal cord with Vaa3D. Each color represents one neuron. c Receptive fields of the 5 sensory neurons innervating hair follicles on digits 1 and 2 (labeled with different colors). The terminals are shown as round dots in c1–c5. d1–d4 Optical slices acquired at different positions along the radial nerve tracing course. Positions are indicated in a. e The topological relationship between the sensory receptive fields on the digits, neuron somas in the DRG and axonal projections in the spinal cord. f Complete tracing courses of the five neurons within the spinal cord. g Axonal projections were mapped to the Allen Spinal Cord Atlas at position C7. h The rostral (upper) and caudal endings (lower) of an axon.

Back to article page