Fig. 4: Cytosol-facing VMAT2YCR captured by the competitive inhibitor RES. | Cell Research

Fig. 4: Cytosol-facing VMAT2YCR captured by the competitive inhibitor RES.

From: Transport and inhibition mechanism for VMAT2-mediated synaptic vesicle loading of monoamines

Fig. 4: Cytosol-facing VMAT2YCR captured by the competitive inhibitor RES.The alternative text for this image may have been generated using AI.

a Binding affinity of VMAT2 variants with RES measured by MST assay, with results summarized below (mean ± SEM, n = 3–4 independent experiments). b RES-bound VMAT2YCR structure (N-domain in cyan and C-domain in yellow-green) captured at cytosol-facing state. An expanded view of the elongated density of RES (purple) is shown on the left. c Cutaway side-view for the overall electronegative vestibule hosting RES molecule. d Residues in close vicinity of RES lining the translocation funnel are detailed, viewed from membrane plane.

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