Fig. 4: LDLR binds directly to Gc of CCHFV.

a Pull-down of CCHFV virions by sohLDLR. CCHFV, biotinylated sohLDLR and magnetic streptavidin beads were co-incubated as indicated. Pull-down was performed with a magnet and the pellet was subjected to RT-qPCR analysis. Data are represented as mean ± SD. b Monoclonal antibodies against CCHFV Gc (ADI 36121), Gn (JE12) or their respective control IgG was immobilized on plates. ELISA-based binding assays were performed with CCHFV, biotinylated sohLDLR and Avidin-HRP. Data are represented as mean ± SD. c Recombinant Gc of CCHFV YL16070 and IbAr 10200 strains and biotinylated sohLDLR were co-incubated as indicated. Pull-down assay was performed with magnetic streptavidin beads and the pellets were subjected to immunoblots with the indicated antibodies. d Biotinylated sohLDLR was immobilized onto the streptavidin biosensors. Binding parameters of recombinant Gc or Gn of the YL16070 and IbAr 10200 strain and VSV-G to LDLR were measured by Bio-Layer Interferometry (BLI) in the indicated PBS buffers. Fitted curves are shown with dotted lines. A 1:1 binding model was used to calculate the K_D.