Fig. 5: Destabilized tetranucleosome impacts the whole structure of chromatin fiber.
From: Structural basis for linker histone H5–nucleosome binding and chromatin fiber compaction
a Decay of product abundance with distance is shown separately for micro-C products with read pairs facing toward OUT-IN and IN-OUT within 1 kb. These plots show the differences in nucleosome interaction frequencies between WT and mutant strains. b Effect of the mutants on interaction frequency at different scales. The color scheme corresponds to the plots in a. These data further illustrate the impact of the histone mutations on chromatin structure. c Data from different strains for a 15 kb locus, with arcs showing interactions between nucleosomes. The interactions represented in white-yellow-red arcs show the interaction intensity for a given pair of loci. d Aggregate plots for nucleosome interaction changes in different mutants compared to WT. e Boxplot showing the micro-C signal intensity for all, down-regulated and up-regulated genes in R119G mutant. f The plot of S20 mid-points of 12× 177 bp nucleosome array in the presence of WT, H2A-G22R, H2B-K116G and H2A-G22R/H2B-K116G with different amounts of histone H1e. g Unfolding Dynamics of Tetranucleosomal Units Analysis in the presence of histones same as f using 4× 177 bp nucleosome array.
