Fig. 7: The GLO2 feedback axis regulates inflammatory responses and immune activation in vitro. | Cell Research

Fig. 7: The GLO2 feedback axis regulates inflammatory responses and immune activation in vitro.

From: Nonenzymatic lysine d-lactylation induced by glyoxalase II substrate SLG dampens inflammatory immune responses

Fig. 7

a Immunoblot detection of indicated proteins and their phosphorylation in tamoxifen-treated BMDMs from Haghfl/fl and Haghfl/flLyz2creERT2 (Hagh−/−) mice. Cells were stimulated by VSV or LPS for indicated time points. b, c GSEA analysis of RNA-seq data of Haghfl/fl and Hagh−/− BMDMs stimulated by VSV for 9 h. d, e ELISA detection of indicated cytokines in medium supernatants of Haghfl/fl and Hagh−/− BMDMs stimulated as indicated. f Immunoblot detection of indicated proteins in Haghfl/fl and Hagh−/− BMDMs stimulated by VSV or LPS for indicated time points. g Immunoblot detection of indicated proteins and their phosphorylation in BMDMs from WT and GLO2 OE mice. Cells were stimulated by VSV or LPS for indicated time points. h, i ELISA detection of indicated cytokines in medium supernatants of WT and GLO2 OE BMDMs stimulated as indicated VSV (h) and LPS (i). j, k Q-PCR analysis of indicated gene expression in WT or GLO2 ARE-sites mutant RAW 264.7 cells stimulated by VSV (9 h) (j) or LPS (2 h) (k). l Cytometric Bead Array System (CBA) detection of indicated cytokines in medium supernatants of human PBMC stimulated by VSV or LPS with 12 h pretreatment of DiFMOC-G (0.4 μM).

Back to article page