Fig. 6: LPHN2 regulates the MET current at the apical surface of utricular hair cells.
From: A force-sensitive adhesion GPCR is required for equilibrioception
a Schematic illustration showing fluid-jet-stimulated MET responses in utricular hair cells before and after treatment with BAPTA, which disrupts the tip links. b Representative MET current traces induced by sinusoidal fluid jet stimulation in cochlear outer hair cells (OHCs) (left panel) or utricular hair cells (right panel) before and after treatment with BAPTA for 5 min (n = 8 per group). The normal-polarity and reverse-polarity MET current traces are colored blue and pink, respectively. The fluid-jet-stimulated cochlear or utricular hair cells are outlined in green. c Quantification of the normal-polarity (outward phase, blue) or reverse-polarity (inward phase, pink) MET current of cochlear (top) or utricular hair cells (bottom) in response to fluid jet stimulation (n = 8 per group). Data are shown as means ± SEM. ***P < 0.001. Data were statistically analyzed using paired two-sided Student’s t-test. d, e Representative current traces (d) and quantitative analysis (e) of fluid-jet-stimulated MET responses in BAPTA-treated utricular hair cells derived from Pc-Lphn2+/+ or Pc-Lphn2fl/fl mice in the absence or presence of 50 nM D11 (n = 10 and 7 for Pc-Lphn2+/+ and Pc-Lphn2fl/fl mice, respectively). Data are shown as means ± SEM. ***P < 0.001; ns no significant difference. Data were statistically analyzed using paired two-sided Student’s t-test. f, g Representative current traces (f) and quantitative analysis (g) of fluid-jet-stimulated MET responses in BAPTA-treated WT utricular hair cells in the absence or presence of 1 μM C14 (n = 5). Data are shown as means ± SEM. **P < 0.01; ns no significant difference. Data were statistically analyzed using paired two-sided Student’s t-test.
