Fig. 4: The effects of Nptx depletion in F-RAM and N-RAM ensembles on the expression of contextual fear memory. | Cell Research

Fig. 4: The effects of Nptx depletion in F-RAM and N-RAM ensembles on the expression of contextual fear memory.

From: Disturbed engram network caused by NPTX downregulation underlies aging-related contextual fear memory deficits

Fig. 4

a, h, o Diagram of AAV injection. b, i, p Experimental scheme of CFC and memory retrieval. c, j, q Representative images of F- and N-RAM engram cells in DG. Green: F-RAM or N-RAM ensemble, EYFP; blue: DAPI. Scale bar, 100 μm. d, e Freezing percentage (d) and discrimination index (e) of WT and Nptx1 cKO mice tested in context A and context C at day 3 (F-RAM). WT, n = 14 mice; Nptx1 cKO, n = 11 mice. f, g Freezing percentage (f) and discrimination index (g) of WT and Nptx2 cKO mice tested in context A and context C at day 3 (F-RAM). WT, n = 14 mice; Nptx2 cKO, n = 13 mice. k, l Freezing percentage (k) and discrimination index (l) of WT and Nptx1 cKO mice tested in context A and context C at day 3 (N-RAM). WT, n = 18 mice; Nptx1 cKO, n = 15 mice. m, n Freezing percentage (m) and discrimination index (n) of WT and Nptx2 cKO mice tested in context A and context C at day 3 (N-RAM). WT, n = 13 mice; Nptx2 cKO, n = 12 mice. r, s Freezing percentage (r) and discrimination index (s) of WT and Nptx1 cKO mice tested in context A and context C at day 3 (F-RAM). WT, n = 11 mice; Nptx1 cKO, n = 11 mice. t, u Freezing percentage (t) and discrimination index (u) of WT and Nptx2 cKO mice tested in context A and context C at day 3 (N-RAM). WT, n = 10 mice; Nptx2 cKO, n = 9 mice. Data are presented as mean ± SEM; *P < 0.05.

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