Fig. 2: AISa-MGs have a distinct transcriptomic profile.
From: The axon initial segment-associated microglia regulate neuronal activity and visual perception
a Schematic of the experimental procedure used to collect cytoplasm and nucleus samples of different groups of MGs. Scale bar, 10 μm. b Expression of homeostatic MG signature genes in different groups of MGs (n = 7, 5, 8, and 5 batches from 3 mice for Ctrl, Soma-only, Soma&AIS, and AIS-only groups, respectively; two-sided Mann–Whitney test). c Heatmap showing upregulated and downregulated genes in different groups of MGs (Z-scored log2(FPKM + 1)). d Venn diagram showing overlaps of upregulated differentially expressed genes among different groups of MGs. e Selected gene ontology (GO) annotations for genes that were similarly upregulated in the AIS-only and Soma&AIS groups of MGs (dotted line, P = 0.05). f Confocal images showing ITGB1 protein expression on the processes of different groups of MGs. Dashed yellow box areas are further magnified on the right. Scale bars, 2 μm and 1 μm. g Quantification of ITGB1 protein expression in different groups of MGs (Ctrl: 0.18 ± 0.02, Soma-only: 0.16 ± 0.01, Soma&AIS: 0.26 ± 0.02, AIS-only: 0.27 ± 0.02; n = 7 mice; two-sided one-way ANOVA). Note that ITGB1 protein was highly expressed on the microglial processes that interacted with neuronal AISs. Error bars indicate SEM.
