Fig. 6: GluA1 expression in the dmPFC promotes T-cell responses in mice within social groups.

a Schematic of the experimental design: social groups (n = 8) consisting of four male GRIA1^−/− littermate mice, one of which received GFP-GluA1 (blue) and three of which received GFP (green), were subjected to the tube test. At the end of the testing period, courtship vocalizations and blood corticosterone were measured, and the mice then received gB vaccination. Sampling time points for FACS analysis are indicated in blue. b Tube-test scores for each test day. Social rank was based on the last 5 test days (yellow). c Percentage of hierarchy stability during the last 5 test days. d Number of courtship vocalizations relative to the group average per rank (left) and comparison between GFP-GluA1-expressing and GFP-expressing mice (right). e Distribution of GFP-GluA1-expressing (blue) or GFP-expressing (green) mice among ranks of social groups. f, g Blood corticosterone levels shown as absolute levels per rank (f), shown relative to the group average per rank (g, left), and compared between GFP-GluA1-expressing and GFP-expressing mice (g, right). h Percentage of lymphocytes in blood leukocytes (top) and percentage of CD8⁺ cells in lymphocytes (bottom) for mice receiving GFP-GluA1 vs GFP. i–k Percentage of gB-specific CD8⁺ T-cells shown as a time course (i), in absolute levels (day 12) (j), and relative to the group average (k) per rank. l, m Percentage of gB-specific CD8⁺ T-cells shown as a time course (l) and relative to the group average (m), comparing GFP-GluA1-expressing vs GFP-expressing mice. n Relative blood corticosterone levels vs relative gB-specific T-cell expansion. Square: rank 1; circle: rank 2; diamond: rank 3; triangle: rank 4. Data are mean ± SEM. *P < 0.05, ****P < 0.0001. Statistics: one-way ANOVA with Tukey’s multiple-comparison test (f, g left, j, k), Pearson’s correlation (e left, n), unpaired Student’s t-test (e right, g right, h, m), and two-way ANOVA with Tukey’s multiple-comparison test (i, l).