Fig. 8: Chemogenetic manipulation of dmPFC neurons influences the activation and gene expression of CD8+ T-cells.
From: Social status impacts T-cell responses through synapse strength in the prefrontal cortex
a Representative image of AAV-mediated hM3Dq-mCherry expression (scale bars: left, 500 µm; right, 25 µm) selectively in the PL-dmPFC of WT mice and schematic of the experimental design with sampling time points indicated in blue. b Representative FACS plots of CD8+ T-cells (in red box) that recognize MHC tetramers loaded with gB peptide at day 12 after vaccination. c Time course of the absolute percentage of gB-specific CD8+ T-cells, showing a comparison between hM3Dq-mCherry (red; n = 22) and mCherry (gray; n = 21) in the dmPFC and relative to the littermate average (day 14). d Volcano plot of differentially expressed genes in splenic CD8+ T-cells at day 14 after vaccination. Data are mean ± SEM. *P < 0.05. Statistics: unpaired Student’s t-test (c, right) and two-way ANOVA with Tukey’s multiple-comparison test (c, left).
