Fig. 7: HBx enhanced CBFβ expression by deubiquitination.

a HepG2 cells were transfected with Flag-HBx expression plasmids and treated with IFN-α as indicated; 36 h later, whole-cell lysates were prepared and immunoblotted with antibodies as indicated. b HepG2 cells were transfected with HBx expression plasmids; 36 h later, whole-cell lysates were subjected to immunoblotting using Flag and GAPDH antibodies, and Q-PCR was performed to analyze the CBFβ mRNA level. c HepG2 cells were transfected with CBFβ or cotransfected with CBFβ and HBx expression plasmids as indicated; 24 h later, cells were treated with MG132 for 8 h; then, whole-cell lysates were prepared and subjected to Co-IP assay, and the blot was immunoblotted with antibodies as indicated. All of the data are from three pooled independent experiments and are shown as the mean ± s.d. ^#p > 0.05