Fig. 1

Rbm47 expression is increased in IL-10-secreting B cells. a IL-10⁺ and IL-10⁻ B cells from LPS-stimulated B cells were isolated by MACS, and the mRNA expression profiles were analyzed via Affymetrix microarrays. The heat map diagram shows the fold change in the expression of different genes related to RNA-binding (only differentially expressed mRNAs with a fold change ≥2.50 or ≤0.40 and P value < 0.05 are included). The red box indicates Rbm47. b, c B cells from splenocytes of Il10-GFP reporter mice were stimulated with or without (Ctrl) 10 μg/ml LPS. On day 3 after stimulation, GFP⁻ and GFP⁺ B cells were sorted via FACS and then subjected to q-PCR (b) or western blot (c) analysis. Numbers indicate the ratio of gray values of the corresponding protein to that of β-actin. Data are representative of at least three independent experiments, and error bars indicate the standard deviation. **P < 0.01; NS not significant