Fig. 2
From: B cell development is critically dependent on NFATc1 activity
Impaired B cell development in Vav-CreNfatc1fl/fl mice. a Photographs of the thymus, spleen, and LNs from Vav-CreNfatc1fl/fl mice compared with littermate Vav-CreNfatc1fl/+ and WT mice. b Total splenocyte numbers in WT (n = 8), Vav-CreNfatc1fl/+ (n = 8), and Vav-CreNfatc1fl/fl (n = 9) mice. c Flow cytometry showing the distribution of CD3+ T and B220+ B cells in the spleen from Vav-CreNfatc1fl/fl mice compared with WT controls. d Absolute numbers of B220+ B cells in the spleen from Vav-CreNfatc1fl/fl (n = 9) mice compared with littermate control (n = 8 for both Vav-CreNfatc1fl/+ and WT) mice. e, f Distribution of IgM+ and IgD+ B cells in the spleen (e) and quantification of the % cells (f) from WT and Vav-CreNfatc1fl/fl mice. g, h Follicular and marginal zone B cell distribution in the spleen (g) and quantification of the percentage of cells (h) from Vav-CreNfatc1fl/fl mice compared with littermate control mice as revealed by CD21 and CD23 staining. i, j Distribution of splenocytes according to CD5 and IgM expression (i) and quantification of the percentage of cells (j) in WT and Vav-CreNfatc1fl/fl mice. k, l Distribution of CD5+ and IgM+ cells (k) and quantification of the percentage of cells (l) in the peritoneum of Vav-CreNfatc1fl/fl mice compared with WT controls. m, n Distribution of follicular and marginal zone B cells (m) and quantification of the percentage of cells (n) in the peritoneum of Vav-CreNfatc1fl/fl mice compared with WT controls. Numbers within each dot plot represent the percentage of the respective population. Data represent one of the three independent experiments (n = 3 per group) and are shown as the mean ± s.d., one-way ANOVA (b, d), and unpaired t-test (f, h, j, l, n)
