Fig. 2 | Cellular & Molecular Immunology

Fig. 2

From: The transcription factor Zfp281 sustains CD4+ T lymphocyte activation through directly repressing Ctla-4 transcription

Fig. 2

TCR-induced activation and cytokine production are decreased in Zfp281-deficient CD4+ T cells. CD25 (a) and CD69 (b) staining on sorted naive CD4+ (CD44loCD62LhiCD4+CD25−) splenocytes from the indicated mice stimulated with the indicated concentration of the anti-CD3 mAb overnight. The numbers above the lines (left) indicate the percentage of CD25+ (a) or CD69+ (b) cells. The statistics of the percentage of CD25+ (a) and CD69+ cells are shown on the right; b the statistics of the percentage of T cells are shown on the left. ELISA detection of IFN-γ (c) and IL-2 (d) secreted by wild-type and Zfp281-deficient naive CD4+ T cells stimulated with the indicated concentration of the anti-CD3 mAb for 3 days. Data are representative of three independent experiments (mean ± s.d.). A two-tailed, unpaired t-test was used. NS not significant. *P < 0.05, **P < 0.01

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