Fig. 3 | Cellular & Molecular Immunology

Fig. 3

From: The transcription factor Zfp281 sustains CD4+ T lymphocyte activation through directly repressing Ctla-4 transcription

Fig. 3

TCR signaling is interrupted in Zfp281-deficient CD4+ T cells. a Immunoblot analysis of total PLC-γ1 and phosphorylated (p-) PLC-γ1 and ZAP-70 in extracts of sorted naive CD4+ T cells from WT and cKO splenocytes that were stimulated for 0, 5, 10, and 20 min with the anti-CD3 mAb and anti-CD28 mAb. b Immunoblot analysis of total and phosphorylated (p-) ERK1/2, JNK and P38 in extracts of cells stimulated as in a. c Calcium flux in naive CD4+ T cells from Zfp281fl/fl and Zfp281fl/flCd4Cre mice after stimulation with anti-CD3 and anti-CD28 mAbs. d Immunoblot analysis of NFAT1 in nuclear extracts of naive CD4+ T cells stimulated as in a. The numbers below the lanes (a, b, d) indicate densitometry analysis. Data are representative of at least three independent experiments

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